윤태현
2018-03-26T08:06:07Z
2018-03-26T08:06:07Z
2014-09
BULLETIN OF THE KOREAN CHEMICAL SOCIETY, 2014, 35(9), P.2625-2629
0253-2964
1229-5949
http://citeseerx.ist.psu.edu/viewdoc/download?doi=10.1.1.861.9480&rep=rep1&type=pdf
http://hdl.handle.net/20.500.11754/52611
Platelets are anuclear discoid-shaped blood cells with key roles in human body. To understand the mechanisms of their activation process, it is required to have analytical imaging techniques capable of acquiring platelet images under fully hydrated conditions. Herein, for the first time, we demonstrate the capability of synchrotronbased transmission X-ray microscopy (TXM) to study platelets (resting and ADP activated) under hydrated and air-dried conditions. To confirm the biological imaging capability of TXM, fixed platelets were imaged and compared with whole mount electron microscopy (EM) images. DM provided morphological information with sufficient spatial resolution with simple and quick sample preparation procedure. We also observed temporal changes during the platelet activation, which initially had a discoid shape (0 s), formed pseudopodia (30 s) and generated a network of fibrin (5 min). Our results clearly demonstrate the potential of TXM technique to study fully hydrated biological samples under in situ conditions.
This research was supported by the Basic Science Research Program (NRF-2012R1A1A2009505) through the National Research Foundation of Korea (NRF) funded by the Korea government Ministry of Science, ICT and Future Planning (MSIP). In addition, this work was supported by MSIP and PAL, XFEL project, Korea. H Nho and HJ Shin also would like to acknowledge the financial support by the Basic Science Research Program (No. 20080062606, CELA-NCRC) through the NRF funded by the Korea government MSIP.
en
WILEY-V C H VERLAG GMBH, BOSCHSTRASSE 12, D-69469 WEINHEIM, GERMANY
Transmission X-ray microscopy
Whole mount electron microscopy
Platelets
Platelet activation
In situ microscopy
GRANULES
SECRETION
RELEASE
Synchrotron-based Transmission X-ray Microscopy (TXM) Observations of Fully Hydrated Blood Platelets and Their Activation Process
Article
35
10.5012/bkcs.2014.35.9.2625
2625-2629
BULLETIN OF THE KOREAN CHEMICAL SOCIETY
Yang, Nuri
Nho, Hyun Woo
Kalegowda, Yogesh
Kim, Jin Bae
Song, Jaewoo
Shin, Hyun-Joon
Yoon, Tae Hyun
2014026628
S
COLLEGE OF NATURAL SCIENCES[S]
DEPARTMENT OF CHEMISTRY
taeyoon