주재범
2018-03-15T06:30:24Z
2018-03-15T06:30:24Z
2014-01
Macromolecular Rapid Communications, Volume 35, Issue 1, Pages 56?65
1521-3927
http://onlinelibrary.wiley.com/doi/10.1002/marc.201300652/full
http://hdl.handle.net/20.500.11754/47230
Anisotropic microarchitectures with different physicochemical properties have been developed as advanced materials for challenging industrial and biomedical applications including switchable displays, multiplexed biosensors and bioassays, spatially-controlled drug delivery systems, and tissue engineering scaffolds. In this study, anisotropic biohybrid microparticles (MPs) spatio-selectively conjugated with two different antibodies (Abs) are first developed for fluorescence-based, multiplexed sensing of biological molecules. Poly(acrylamide-co-acrylic acid) is chemically modified with maleimide- or acetylene groups to introduce different targeting biological moieties into each compartment of anisotropic MPs. Modified polymer solutions containing two different fluorescent dyes are separately used for electrohydrodynamic co-jetting with side-by-side needle geometry. The anisotropic MPs are chemically stabilized by thermal imidization, followed by bioconjugation of two different sets of polyclonal Abs with two individual compartments via maleimide-thiol coupling reaction and Huisgen 1,3-dipolar cycloaddition. Finally, two compartments of the anisotropic biohybrid MPs are spatio-selectively associated with the respective monoclonal Ab-immobilized substrate in the presence of the antigen by sandwich-type immunocomplex formation, resulting in their ordered orientation due to the spatio-specific molecular interaction, as confirmed by confocal laser scanning microscopy. In conclusion, anisotropic biohybrid MPs capable of directional binding have great potential as a new fluorescence-based multiplexing biosensing system.
Funded by Ministry of Education, Science, and Technology, Korea. Grant Numbers: 2010?0013284, 2012R1A1A2043792, 2012?0009838, R11?2008?044?01002?0, Ministry of Science, ICT and Future Planning, Korea. Grant Number: 2013R1A2A2A04016796, Agency for Defense Development
en
WILEY-BLACKWELL, 111 RIVER ST, HOBOKEN 07030-5774, NJ USA
anisotropy
biohybrid microparticles
electrohydrodynamic co-jetting
multiplexed biosensing
sandwich immunocomplex
Synthesis, Characterization, and Directional Binding of Anisotropic Biohybrid Microparticles for Multiplexed Biosensing
Article
35
10.1002/marc.201300652
56-65
MACROMOLECULAR RAPID COMMUNICATIONS
Jung, Woo
Jalani, Ghulam
Ko, Juhui
Choo, Jaebum
Lim, Dong Woo
2014035552
S
GRADUATE SCHOOL[S]
DEPARTMENT OF BIONANOTECHNOLOGY
jbchoo