전대원
2018-03-12T04:05:57Z
2018-03-12T04:05:57Z
2013-12
Journal of Gastroenterology and Hepatology, Vol.28, No.12 [2013], p1877-1884
0815-9319
http://onlinelibrary.wiley.com/doi/10.1111/jgh.12317/abstract?
http://hdl.handle.net/20.500.11754/45240
Background and AimSeveral epidemiological studies have shown that coffee intake attenuates the progression of liver fibrosis; however, the mechanism is unclear.AimsWe investigated the direct effects of caffeine on hepatic stellate cells (HSCs) and assessed whether caffeine attenuated intrahepatic fibrosis in rat model of liver cirrhosis.MethodsHuman hepatic stellate cell line, an immortalized human HSCs line, was used in in vitro assay system. Cell migration and proliferation were assessed in presence of various caffeine concentrations (0, 1, 5, and 10mmol), and levels of procollagen type Ic and -smooth muscle actin (-SMA) were measured by Western blot. Severity of liver inflammation and fibrosis were compared between thioacetamide-treated rats with and without caffeine supplementation.ResultsCaffeine increased HSCs apoptosis and intracellular F-actin and cyclic adenosine monophosphate expression. Caffeine also inhibited procollagen type Ic and -SMA expression in a dose- and time-dependent manner. In rat model, caffeine decreased periportal inflammation, levels of inflammatory cells (1.40.52 vs 2.6 +/- 0.46, P<0.05), and fibrosis (2.1 +/- 0.35 vs 2.9 +/- 0.84, P<0.05). Transforming growth factor- and -SMA expressions were also reduced by caffeine.ConclusionCaffeine attenuates the progression of liver fibrosis by inhibiting HSCs adhesion and activation.
This work was supported by the research fund of National Research Foundation of Korea 2011-0007127.
en
Blackwell Publishing Ltd
adhesion
caffeine
liver fibrosis
stellate cell
Caffeine attenuates liver fibrosis via defective adhesion of hepatic stellate cells in cirrhotic model
Article
12
28
10.1111/jgh.12317
1877-1884
JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY
Shim, S. G.
Jun, D. W.
Kim, E. K.
Saeed, W. K.
Lee, K. N.
Lee, H. L.
Lee, O. Y.
Choi, H. S.
Yoon, B. C.
2013010693
S
COLLEGE OF MEDICINE[S]
DEPARTMENT OF MEDICINE
noshin
55095317700