박진구
2018-03-29T09:03:09Z
2018-03-29T09:03:09Z
2013-09
Journal of Biotechnology, 2013, 168(4), P.584-588
0168-1656
https://www.sciencedirect.com/science/article/pii/S0168165613003805
http://hdl.handle.net/20.500.11754/53934
We have reported that nanowell array (NWA) can enhance electrochemical detection of molecular binding events by controlling the binding sites of the captured molecules. Using NWA biosensor based amperometric analysis, we have detected biological macromolecules such as DNA, protein or aptamers at low concentrations. In this research, we developed an impedimetric immunosensor based on wafer-scale NWA for electrochemical detection of stress-induced-phosphoprotein-1 (STIP-1). In order to develop NWA sensor through the cost-effective combination of high-throughput nanopattern, the NWA electrode was fabricated on Si wafer by krypton-fluoride (KrF) stepper semiconductor process. Finally, 12,500,000 ea nanowell with a 500 nm diameter was fabricated on 4 mm × 2 mm substrate. Next, by using these electrodes, we measured impedance to quantify antigen binding to the immunoaffinity layer. The limit of detection (LOD) of the NWA was improved about 100-fold compared to milli-sized electrodes (4 mm × 2 mm) without an NWA. These results suggest that wafer-scale NWA immunosensor will be useful for biosensing applications because their interface response is appropriate for detecting molecular binding events.
This research was supported by the Basic Science Research Program of the National Research Foundation of Korea (NRF), funded by the Ministry of Education, Science and Technology (2008-0061862). And also supported by the National Science Foundation Nanoscale Science and Engineering Center (NSEC) for High-Rate Nanomanufacturing (grant no. 0832785).
en
Elsevier Science B.V
Nanowell arrays (NWA) immunosensor
Wafer-scale nanopatterning
Electrochemical impedance spectroscopy (EIS)
Stress-induced-phosphoprotein-1 (STIP-1)
Wafer-scale nanowell array patterning based electrochemical mpedimetric immunosensor
Article
168
10.1016/j.jbiotec.2013.08.029
584-588
JOURNAL OF BIOTECHNOLOGY
Lee, Ju-Kyung
Busnaina, Ahmed
Lee, Hea-Yeon
Cho, Si-Hyeong
Lee, Jung-Hwan
Ryu, Heon-Yul
Lim, Sun-Hee
Oh, Byung-Do
Lee, Chang-Woo
Park, Jin-Goo
2013010524
S
GRADUATE SCHOOL[S]
DEPARTMENT OF BIONANOTECHNOLOGY
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