성일훈
2018-03-13T00:30:45Z
2018-03-13T00:30:45Z
2013-05
Joint Bone Spine, 2013, 80(3), P.307-314
1297-319X
https://www.sciencedirect.com/science/article/pii/S1297319X12002163?via%3Dihub
Objective1,25-dihydroxyvitamin D3 (1,25(OH)2D3) is a key molecule to maintain calcium homeostasis and bone metabolism. It was recently reported that 1,25(OH)2D3 directly inhibited osteoclast differentiation in mouse bone marrow cells and human bone marrow-derived colony-forming unit granulocyte macrophage (CFU-GM) cells. However, the direct effects of 1,25(OH)2D3 and its affecting mechanisms on the osteoclast differentiation of human osteoclast precursors remain largely unknown. In this study, we examined the direct effects of 1,25(OH)2D3 on the osteoclastogenesis of human peripheral blood (PB) osteoclast precursors.MethodsIn vitro osteoclastogenesis assays were performed using osteoclast precursors from normal PB. The gene expressions were analyzed using real-time PCR. The cell surface proteins, including c-Fms and RANK, were measured by flow cytometry.Results1,25(OH)2D3 strongly inhibited osteoclast differentiation and it suppressed the expression of RANK in the human PB osteoclast precursors. One mechanism of RANK inhibition by 1,25(OH)2D3 is down-regulation of the M-CSF receptor c-Fms, which is required for the expression of RANK. In contrast to the previous reports on mouse osteoclast precursors, 1,25(OH)2D3 did not affect the expression of c-Fos. Parallel to the inhibition of osteoclastogenesis, 1,25(OH)2D3 increased the expression and phosphorylation of CCAAT enhancer-binding protein β (C/EBPβ), which is a recently discovered inhibitor of osteoclastogenesis.ConclusionsOur results show that 1,25(OH)2D3 inhibits human osteoclastogenesis by decreasing the RANK+ osteoclast precursors, and we suggest that 1,25(OH)2D3 may be a powerful therapeutic agent for treating inflammation-induced bone disease that shows excessive osteoclast activation.
This work was supported by the National Natural Science Foundation of P. R. China (Grants No. 31160500), the Young scientists training plan of Jiangxi province (Grant No. 20112BCB23030), the International cooperation plan of Jiangxi province (Grant No. 20111BDH80036) and Foundation of Sichuan Province (2011NZ0099-6).
en
Elsevier B.V
1,25-dihydroxyvitamin D-3 inhibits directly human osteoclastogenesis by down-regulation of the c-Fms and RANK expression
Article
3
80
10.1016/j.jbspin.2012.09.011
307-314
JOINT BONE SPINE
Kim, Tae-Hwan
Lee, Bitnara
Kwon, Eunji
Choi, Choong Hyeok
Sung, Il-Hoon
Kim, Yongjin
Sohn, Jeongwon
Ji, Jong Dae
2013010440
S
COLLEGE OF MEDICINE[S]
DEPARTMENT OF MEDICINE
sungih