민경환
2018-02-27T06:35:05Z
2018-02-27T06:35:05Z
2016-03
QJM-AN INTERNATIONAL JOURNAL OF MEDICINE, v. 109, NO 3, Page. 167-173
1460-2725
1460-2393
https://academic.oup.com/qjmed/article/109/3/167/1854947
http://hdl.handle.net/20.500.11754/40859
Background: The importance of sensitive methods for the detection of epidermal growth factor receptor (EGFR) mutation is emphasized. The aim of this study is to perform comparative and concordance analyses of direct sequencing, pyrosequencing and peptide nucleic acid (PNA) clamping for detecting EGFR gene mutations using archived tissue and cytology specimens. Methods: Samples from a total of 112 cases, which were diagnosed with adenocarcinoma of the lung at nine hospitals in Korea were collected. Using the above three methods, the concordance rates of EGFR mutations in exons 18, 19, 20 and 21 were analysed and validated in comparative tissue and cytology specimens. Results: Comparison of EGFR mutation detection between the tissue and cytology had a high concordance rate. The diagnostic performance of pyrosequencing and PNA clamping in tissue was higher than that of direct sequencing as well as cytology. Additionally, among some of the patients who had EGFR wild type by single method, EGFR mutations were detected by other methods. Cytology specimens had a diagnostic performance for the detection of EGFR mutations. Conclusions: Cytology specimens had a diagnostic performance for the detection of EGFR mutations that was comparable to that of tissues. For detecting EGFR mutations, pyrosequencing or PNA clamping was more sensitive than direct sequencing. In EGFR mutation negative patients who are difficult to obtain tissue, repeating test using pyrosequencing or PNA clamping is recommended to improve the detection rate of EGFR mutation than only one, especially in cytology.
This research was conducted with support of AstraZeneca (ISSIRES0079). This study was conducted under the approval of the Institutional Review Board of the Konkuk University Medical Center (KUH 1210016).
en
OXFORD UNIV PRESS
GROWTH-FACTOR RECEPTOR
POLYMERASE-CHAIN-REACTION
NEEDLE-ASPIRATION
KRAS MUTATIONS
CANCER
EFFUSION
IMMUNOCYTOCHEMISTRY
GEFITINIB
CARCINOMA
THERAPY
Comparison of EGFR mutation detection between the tissue and cytology using direct sequencing, pyrosequencing and peptide nucleic acid clamping in lung adenocarcinoma: Korean multicentre study
Article
10.1093/qjmed/hcv103
QJM-AN INTERNATIONAL JOURNAL OF MEDICINE
Min, Kyueng-Whan
Kim, Wan-Seop
Jang, Se Jin
Choi, Yoo Duk
Chang, Sunhee
Jung, Soon Hee
Kim, Lucia
Roh, Mee Sook
Lee, Choong Sik
Shim, Jung Weon
2016002804
S
COLLEGE OF MEDICINE[S]
DEPARTMENT OF MEDICINE
kyueng