주재범
2017-11-16T01:27:16Z
2017-11-16T01:27:16Z
2016-01
BIOSENSORS & BIOELECTRONICS, v. 78, Page. 530-537
0956-5663
1873-4235
http://www.sciencedirect.com/science/article/pii/S0956566315306503?via%3Dihub
http://hdl.handle.net/20.500.11754/31445
User-friendly lateral flow (LF) strips have been extensively used for point-of-care (POC) self-diagnostics, but they have some limitations in their detection sensitivity and quantitative analysis because they only identify the high cut-off value of a biomarker by utilizing color changes that are detected with the naked eye. To resolve these problems associated with LF strips, we developed a novel surface-enhanced Raman scattering (SERS)-based LF assay for the quantitative analysis of a specific biomarker in the low concentration range. Herein, human immunodeficiency virus type 1 (HIV-1) DNA was chosen as the specific biomarker. Raman reporter-labeled gold nanoparticles (AuNPs) were employed as SERS nano tags for targeting and detecting the HIV-1 DNA marker, as opposed to using bare AuNPs in LF strips. It was possible to quantitatively analyze HIV-1 DNA with high sensitivity by monitoring the characteristic Raman peak intensity of the DNA-conjugated AuNPs. Under optimized conditions, the detection limit of our SERS-based lateral flow assay was 0.24 pg/mL, which was at least 1000 times more sensitive compared to colorimetric or fluorescent detection methods. These results demonstrate the potential feasibility of the proposed SERS-based lateral flow assay to quantitatively detect a broad range of genetic diseases with high sensitivity. (C) 2015 Elsevier B.V. All rights reserved.
The National Research Foundation of Korea supported this work through Grant numbers 2008-0061891 and 2009-00426. The Nano Material Technology Development Program also supported this work, through the National Research Foundation of Korea, funded by the Ministry of Science, ICT and Future Planning (2012M3A7B4035288). This work was partially supported by a grant of the National Natural Science Foundation of China through Grant number 21275158.
en
ELSEVIER ADVANCED TECHNOLOGY
Human immunodeficiency virus type 1 (HIV-1)
Surface-enhanced Raman scattering
Gold nanoparticles
DNA
Lateral flow assay
A SERS-based lateral flow assay biosensor for highly sensitive detection of HIV-1 DNA
Article
78
10.1016/j.bios.2015.11.099
530-537
BIOSENSORS & BIOELECTRONICS
Fu, Xiuli
Cheng, Ziyi
Yu, Jimin
Choo, Priscilla
Chen, Lingxin
Choo, Jaebum
2016001453
S
GRADUATE SCHOOL[S]
DEPARTMENT OF BIONANOTECHNOLOGY
jbchoo