배옥남
2018-02-05T02:11:09Z
2018-02-05T02:11:09Z
2015-01
KIDNEY & BLOOD PRESSURE RESEARCH, v. 40, No. 1, Page. 1-12
1420-4096
1423-0143
https://www.karger.com/Article/Abstract/368466
http://hdl.handle.net/20.500.11754/35383
Background/Aims: Drug-induced acute kidney injury (AKI) has been a severe threat to hospitalized patients, raising the urgent needs to develop strategies to reduce AKI. We investigated the protective activity of Dendropanax morbifera (DP), a medicinal plant which has been widely used to treat infectious and pain diseases, on acute kidney injury (AKI) using cisplatin-induced nephropathic models. Methods: Both in vitro renal tubular cells (NRK-52E) and in vivo rat models were used to demonstrate the nephroprotective effect of DP. Results: Methanolic extract from DP significantly reduced cisplatin-induced toxicity in renal tubular cells. Through successive liquid extraction, the extract of DP was separated into n-hexane, CHCl3, EtOAc, n-BuOH, and H2O fractions. Among these, the CHCl3 fraction (DPCF) was found to be most potent. The protective activity of DPCF was found to be mediated through anti-oxidant, mitochondrial protective, and anti-apoptotic activities. In in vivo rat models of AKI, treatment with DPCF significantly reversed the cisplatin-induced increase in blood urea nitrogen and serum creatinine and histopathologic damage, recovered the level of anti-oxidant enzymes, and inhibited renal apoptosis. Conclusion: We demonstrated that DP extracts decreased cisplatin-induced renal toxicity, indicating its potential to ameliorate drug-associated acute kidney damage.
This work was supported by a grant (No. C0036782) from Business for Cooperative R&D between Industry, Academy, and Research Institute funded Korea Small and Medium Business Administration in 2012, and by a study (No. 2013-A-7184-010203) on the Leaders INdustry-university Cooperation Project supported by the Ministry of Education (MOE), Korea.
en_US
KARGER
Nephrotoxicity
Cisplatin
Acute kidney injury
Dendropanax morbifera
ACUTE TUBULAR-NECROSIS
ANTICOMPLEMENT ACTIVITY
INDUCED NEPHROTOXICITY
CELL-DEATH
MECHANISMS
LEVEILLE
RATS
TRANSPORTERS
QUERCETIN
TOXICITY
Protective Activity of Dendropanax Morbifera Against Cisplatin-Induced Acute Kidney Injury
Article
1
40
10.1159/000368466
1-12
KIDNEY & BLOOD PRESSURE RESEARCH
Kim, Eun-Sun
Lee, Jong Soo
Akram, Muhammad
Kim, Kyeong-A
Shin, Young-Jun
Yu, Jong-Hyun
Bae, Ok-Nam
2015002413
E
COLLEGE OF PHARMACY[E]
DEPARTMENT OF PHARMACY
onbae