황승용
2017-05-25T02:11:46Z
2017-05-25T02:11:46Z
2015-09
BIOCHIP JOURNAL, v. 9, NO 3, Page. 259-267
1976-0280
2092-7843
https://link.springer.com/article/10.1007/s13206-015-9310-4
http://hdl.handle.net/20.500.11754/27442
Humans can absorb volatile organic compounds (VOCs), such as ethylbenzene (EB), via inhalation resulting in various effects such as hepatotoxicity and even carcinogenicity. Because occupational EB exposure occurs frequently in the synthetic rubber and plastic industry, its toxicity has been previously reported by various in vitro as well as in vivo animal studies. EB has also been classified as a group 2B possible carcinogen by the IARC. However, these studies investigated the toxicity of EB used a much higher dose than expected occupational exposure. Thus the results are likely to be far away from to be the basis for the validation of the toxicity of the EB exposure. Because the previous studies examine only phenotypes, it is still unclear what and how EB affects physiological pathways in the human body. Using microarray platform, we investigated the gene expression profiles and genomic methylation patterns of 66 human blood samples from employees of local plants that use EB. The affected genes were functionally analyzed using the DAVID Tool and IPA. The methylation array demonstrated that, compared to the control group, a total of 1446 genes were hypermethylated and 60 genes were hypomethylated. Among the 378 differentially expressed genes, 12 had proper epigenetic correlation with the altered methylation patterns. Our study demonstrated how EB exposure affects the human genome by investigating the gene expression profiles and DNA methylation profiles of blood cells. We expect that our results will help establish the genomic basis of the effect of EB exposure on humans.
The samples were given by Korea University Medical Center (IRB #AS 14039) and this work was supported by the Korea Ministry of Environment as a "Converging technology project" (Project No. 201400165002). Microarray platforms were provided by BioCore Co., Ltd (Seoul, Korea).
en
KOREAN BIOCHIP SOCIETY-KBCS
Volatile organic compounds (VOCs)
Ethylbenzene (EB)
Gene expression profiling
DNA methylation profiling
Microarray
Integrative Analyses of Differential Gene Expression and DNA Methylation of Ethylbenzene-exposed Workers
Article
3
9
10.1007/s13206-015-9310-4
259-267
BIOCHIP JOURNAL
Kim, Gi Won
Hong, Ji Young
Yu, So-Yeon
Ahn, Jeong Jin
Kim, Youngjoo
Son, Sang Wook
Park, Jong-Tae
Hwang, Seung Yong
2015006188
S
GRADUATE SCHOOL[S]
DEPARTMENT OF BIONANOTECHNOLOGY
syhwang