설원제
2019-08-06T04:46:15Z
2019-08-06T04:46:15Z
2019-02
CANCERS, v. 11, NO 2, no. 200
2072-6694
https://www.mdpi.com/2072-6694/11/2/200
https://repository.hanyang.ac.kr/handle/20.500.11754/108272
The separation of circulating tumor cells (CTCs) from the peripheral blood is an important issue that has been highlighted because of their high clinical potential. However, techniques that depend solely on tumor-specific surface molecules or just the larger size of CTCs are limited by tumor heterogeneity. Here, we present a slanted weir microfluidic device that utilizes the size and deformability of CTCs to separate them from the unprocessed whole blood. By testing its ability using a highly invasive breast cancer cell line, our device achieved a 97% separation efficiency, while showing an 8-log depletion of erythrocytes and 5.6-log depletion of leukocytes. We also developed an image analysis tool that was able to characterize the various morphologies and differing deformability of the separating cells. From the results, we believe our system possesses a high potential for liquid biopsy, aiding future cancer research.
This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF), funded by the Ministry of Science, ICT, and Future Planning (NRF-2017R1A2B4003189), and the Ministry of Education of Korea (NRF-2012R1A6A1029029).
en
MDPI
circulating tumor cell
cell separation
microfluidics
Continuous Separation of Circulating Tumor Cells from Whole Blood Using a Slanted Weir Microfluidic Device
Article
2
11
10.3390/cancers11020200
200-200
CANCERS
Yoon, Yousang
Lee, Jusin
Ra, Moonsoo
Gwon, Hyeokshin
Lee, Seungwon
Kim, Min Young
Yoo, Ki-Chun
Sul, Onejae
Kim, Chul Geun
Kim, Whoi-Yul
2019045118
S
RESEARCH INSTITUTE[S]
INSTITUTE OF NANO SCIENCE AND TECHNOLOGY
ojsul
U-9544-2019