심지원
2019-12-02T20:18:13Z
2019-12-02T20:18:13Z
2017-12
MOLECULES AND CELLS, v. 40, no. 12, page. 976-985
1016-8478
0219-1032
http://www.molcells.org/journal/view.html?doi=10.14348/molcells.2017.0287
https://repository.hanyang.ac.kr/handle/20.500.11754/116493
Iron is an essential divalent ion for aerobic life. Life has evolved to maintain iron homeostasis for normal cellular and physiological functions and therefore imbalances in iron levels exert a wide range of consequences. Responses to iron dysregulation in blood development, however, remain elusive. Here, we found that iron homeostasis is critical for differentiation of Drosophila blood cells in the larval hematopoietic organ, called the lymph gland. Supplementation of an iron chelator, bathophenanthroline disulfate (BPS) results in an excessive differentiation of the crystal cell in the lymph gland. This phenotype is recapitulated by loss of Fer1HCH in the intestine, indicating that reduced levels of systemic iron enhances crystal cell differentiation. Detailed analysis of Fer1HCH-taggedGFP revealed that Fer1HCH is also expressed in the hematopoietic systems. Lastly, blocking Fer1HCH expression in the mature blood cells showed marked increase in the blood differentiation of both crystal cells and plasmatocytes. Thus, our work suggests a relevance of systemic and local iron homeostasis in blood differentiation, prompting further investigation of molecular mechanisms underlying iron regulation and cell fate determination in the hematopoietic system.
This work was supported by the National Research Foundation (NRF) grant funded by the Korean government (NRF-2017R1C1B2007343) and the research fund of Hanyang University (HY-2014-N) to J. S.
en_US
KOREAN SOC MOLECULAR & CELLULAR BIOLOGY
blood
BPS
crystal cell
Drosophila
ferritin
Fer1HCH
FAC
hemocyte
intestine
iron
lymph gland
plasmatocyte
Iron homeostasis controls myeloid blood cell differentiation in Drosophila
Article
12
40
10.14348/molcells.2017.0287
976-985
MOLECULES AND CELLS
Yoon, Sunggyu
Cho, Bumsik
Shin, Mingyu
Koranteng, Ferdinand
Cha, Nuri
Shim, Jiwon
2017002526
S
COLLEGE OF NATURAL SCIENCES[S]
DEPARTMENT OF LIFE SCIENCE
jshim