채필석
2019-11-21T00:08:11Z
2019-11-21T00:08:11Z
2017-02
CHEMICAL SCIENCE, v. 8, no. 2, page. 1169-1177
2041-6520
2041-6539
https://pubs.rsc.org/en/content/articlelanding/2017/SC/C6SC02981G#!divAbstract
https://repository.hanyang.ac.kr/handle/20.500.11754/112923
Amphiphile selection is a crucial step in membrane protein structural and functional study. As conventional detergents have limited scope and utility, novel agents with enhanced efficacy need to be developed. Although a large number of novel agents have been reported, so far there has been no systematically designed comparative study of the protein stabilization efficacy of stereo-isomeric amphiphiles. Here we designed and prepared a novel class of stereo-isomeric amphiphiles, designated butane-1,2,3,4-tetraolbased maltosides (BTMs). These stereoisomers showed markedly different behaviour for most of the targeted membrane proteins depending on the chirality of the linker region. These findings indicate an important role for detergent stereochemistry in membrane protein stabilization. In addition, we generally observed enhanced detergent efficacy with increasing alkyl chain length, reinforcing the importance of the balance between hydrophobicity and hydrophilicity in detergent design. The stereo-isomeric difference in detergent efficacy observed provides an important design principle for the development of novel amphiphiles for membrane protein manipulation.
This work was supported by the National Research Foundation of Korea (NRF) funded by the Korean government (MSIP) (grant number 2008-0061891 and 2016R1A2B2011257 to P. S. C. and M. D.). The work was also supported by Biotechnology and Biological Sciences Research Council grant BB/K017292/1 to B. B. and by the Danish Council for Independent Research Sapere Aude program 0602-02100B (to C. J. L.).
en_US
ROYAL SOC CHEMISTRY
Butane-1,2,3,4-tetraol-based amphiphilic stereoisomers for membrane protein study: importance of chirality in the linker region
Article
2
8
10.1039/c6sc02981g
1169-1177
CHEMICAL SCIENCE
Das, Manabendra
Du, Yang
Ribeiro, Orquidea
Hariharan, Parameswaran
Guan, Lan
Loland, Claus J.
Kobilka, Brian K.
Chae, Pil Seok
2017003057
S
GRADUATE SCHOOL[S]
DEPARTMENT OF BIONANOTECHNOLOGY
pchae
http://orcid.org/0000-0003-1799-3304