송충길
2019-04-11T01:06:14Z
2019-04-11T01:06:14Z
2016-12
BIOSENSORS & BIOELECTRONICS, v. 86, Page. 548-556
0956-5663
1873-4235
https://www.sciencedirect.com/science/article/pii/S0956566316306819?via%3Dihub
https://repository.hanyang.ac.kr/handle/20.500.11754/101684
A label-free immunosensor based on electrochemical impedance spectroscopy has been developed for the sensitive detection of a cardiac biomarker myoglobin (cMyo). Hydrothermally synthesized graphene quantum dots (GQDs) have been used as an immobilized template on screen printed electrodes for the construction of an impedimetric sensor platform. The GQDs-modified electrode was conjugated with highly specific anti-myoglobin antibodies to develop the desired immunosensor. The values of charge transfer resistance (R-ct) were monitored as a function of varying antigen concentration. The R-ct value of the immunosensor showed a linear increase (from 0.20 to 0.31 k Omega) in the range of 0.01-100 ng/mL cMyo. The specific detection of cMyo was also made in the presence of other competing proteins. The limit of detection for the proposed immunosensor was, estimated as 0.01 ng/mL which is comparable to the standard ELISA techniques. (C) 2016 Elsevier B.V. All rights reserved.
The present research work has been funded through CSIR, India grant (OMEGA/PSC0202/2.2.5) from CSIR, India. Satish Kumar Tuteja also thanks the CSIR, India his senior research fellowship. We are grateful to the Director, CSIR-CSIO, Chandigarh, India.
en
ELSEVIER ADVANCED TECHNOLOGY
Graphene quantum dots
Myoglobin
Electrochemical Impedance Spectroscopy
Label-free immunosensor
Cardiac marker
A label-free electrochemical immunosensor for the detection of cardiac marker using graphene quantum dots (GQDs)
Article
86
10.1016/j.bios.2016.07.052
548-556
BIOSENSORS & BIOELECTRONICS
Tuteja, Satish K.
Chen, Rui
Kukkar, Manil
Song, Chung Kil
Mutreja, Ruchi
Singh, Suman
Paul, Ashok K.
Lee, Haiwon
Kim, Ki-Hyun
Deep, Akash
2016001453
S
RESEARCH INSTITUTE[S]
INSTITUTE OF NANO SCIENCE AND TECHNOLOGY
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